SPECTRAL Lago & Lago X

The SPECTRAL Lago and Lago X (x-ray imaging) optical imaging systems provide a powerful in vivo imaging system in a user friendly unit with the largest field of view on the market. The Lago and Lago X imaging systems are designed from the ground up using modern components to provide a stable, high quality package for optical preclinical imaging. The Lago and Lago X are designed around a rigid “strong back” backbone  that serves as an “optical bench” coupling the sample and all imaging components together in a stable spatial configuration. This structural integrity ensures highly accurate and extremely reproducible images and calibrations, day in and day out and year after year.  Since the Lago and Lago X imaging systems include all the features available from SI Imaging, they are a perfect choice for the flexibility required by an imaging core facility.

  • Some of the features which make the Lago and Lago X unique and the best solution for an optical imaging solution:
  • Fluorescence excitation is provided by a patented LED based illumination that eliminates the background inherent in white light based excitation systems.  The Lago imaging system contains all 20 excitation filters at all times allowing for its use in the demanding imaging core environment.
  • Ergonomics are addressed with the vertically opening access door, minimizing the system’s footprint and avoiding interference with surrounding equipment or personnel in crowded laboratory situations.  The vertical opening door also improves waste anesthesia gas control. 
  • The Lago and Lago X allow the use of any commercials available gas anesthesia system for preclinical in vivo imaging.
  • The Lago and Lago X high performance CCD cameras do not require liquid cooling minimizing failures and leaks.
  • The Lago can be upgraded to a Lago X to perform x-ray imaging for small animals and 50kV maximum x-ray energy allows for larger animals such as rats and guinea pigs.

While SI Imaging systems can be used for a wide variety of applications, including in vivo and in vitro molecular imaging, purchasers should be aware that some methods and applications for molecular imaging may be subject to various patent rights.

Patented Excitation (Illumination) LED Wavelengths

360, 405, 430, 465, 500, 535, 570, 605, 640, 675, 710, 745, 770 and 805nm

Emission Filters
490, 510, 530, 550, 570, 590, 610, 630, 650, 670, 690, 710, 730, 750, 770, 790, 810, 830, 850, and 870nm
A specialized filter is available for plant imaging.

View the Lago and Lago X Brochure

  • Fluorescent Imaging
    • SI Imaging’s unique patented, 14 LED array used for the Lago and Lago X optical imaging systems’ fluorescence excitation source provides bright stable fluorescence illumination with tightly controlled wavelengths. The issues of maintenance, out of band blocking and calibration stability associated with lamp-based systems, are all eliminated by this advanced technology. The automated emission filter system contains 20 filters at all times ensuring the SPECTRAL Lago is ready for any imaging task. The SPECTRAL Lago is designed to employ an extended LED range to accommodate the modern NIR probes. Filters used in all SI Imaging optical imaging systems system employ the most advanced technologies to provide extremely clean and efficient wavelength transmission in the desired range. These high quality filters, coupled with the extremely narrow emission of the LED based system, virtually eliminate problems with out of band transmission. Housing the complete set of emission filters is often required for imaging cores.

      Patented Excitation (Illumination) LED Wavelengths
      360, 405, 430, 465, 500, 535, 570, 605, 640, 675, 710, 745, 770 and 805nm

      Emission Filters
      490, 510, 530, 550, 570, 590, 610, 630, 650, 670, 690, 710, 730, 750, 770, 790, 810, 830, 850, 870nm.
      A specialized filter is available for plant imaging.

  • Luminescent Imaging
    • Luminescence imaging is one of the most challenging imaging modalities, due to the relatively weak light emission. Due to the deeply cooled cameras, SPECTRAL ultra-low noise images are collected for times ranging up to many minutes, if necessary, allowing Lago and Lago X optical imaging systems to provide the highest signal to noise ratio achievable. The large format CCD and state of the art optics provide high quality images with large fields of view, while maintaining efficient light collection across the entire image. SPECTRAL Lago optical imaging systems all include a custom-built air-cooled camera, thus avoiding the complexities and expense associated with liquid cooled systems.

  • X-ray Imaging
    • A 50 kV variable energy x-ray source is located in the bottom of the imaging chamber for x-ray imaging of samples positioned near the top of the chamber, where a state of the art Time Delay Integration (TDI) sensor resides. This sensor is a line scan design that moves rapidly across the entire optical image field to provide a co-registered x-ray image that can be used for optical overlays. In the SPECTRAL Lago imaging system, the x-ray source translates with the sensor to reduce x-ray dose and spatial error. This high resolution imaging technology is increasingly used in digital x-ray imaging in all applications where a fast, large field of view, high-resolution image is required. The Lago X optical imaging system brings x-ray imaging capability to you in an easy to use, versatile package, ideally suited for laboratory use. The entire x-ray imaging option is fully automated, integrating into the software package, and easily used by researchers of any background, not only imaging specialists.

  • System Integration
    • Lago and Lago X optical imaging systems tightly integrate all imaging modalities into one easy to use, highly automated package. The system is designed with simplicity and functionality in mind, so that researchers need not be imaging experts to get accurate, quantitative results. SPECTRAL Lago optical imaging systems are factory calibrated to provide NIST traceable absolute optical intensity measurements. These optical images can be co-registered with optical or photographic images to provide source location as well as strength. Images are automatically corrected for dark current, providing a final image that is precisely what the researcher needs. The user-friendly AMIView software package allows users to rapidly collect and store images for post-imaging analysis, either on the systems own computer or on the user’s desktop computer (Macintosh or Windows) after the imaging session is complete. Analysis copies of AMIView software are free of charge to users.

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* Cerenkov imagery provided by and credited to Van Andel Research Institute